3VPP

Crystal Structure of the Human CLEC9A C-type Lectin-Like Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1271
Spacegroup name	P 1 21 1
Unit cell lengths	42.170, 53.824, 57.446
Unit cell angles	90.00, 109.35, 90.00

Refinement procedure

Resolution	19.894 - 1.642
R-factor	0.1908
Rwork	0.189
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fm5
RMSD bond length	0.007
RMSD bond angle	1.122
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.740
High resolution limit [Å]	1.640	4.800	1.640
Rmerge	0.114	0.067	0.765
Number of reflections	56639
<I/σ(I)>	8.33	17.84	2.1
Completeness [%]	97.2	98.2	93.5
Redundancy	3.81	3.86	3.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	310	30% PEG 8000, 0.2M MgCl2, 0.1M Tris pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K