3VPP
Crystal Structure of the Human CLEC9A C-type Lectin-Like Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1271 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.170, 53.824, 57.446 |
Unit cell angles | 90.00, 109.35, 90.00 |
Refinement procedure
Resolution | 19.894 - 1.642 |
R-factor | 0.1908 |
Rwork | 0.189 |
R-free | 0.22650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fm5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.122 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 1.740 |
High resolution limit [Å] | 1.640 | 4.800 | 1.640 |
Rmerge | 0.114 | 0.067 | 0.765 |
Number of reflections | 56639 | ||
<I/σ(I)> | 8.33 | 17.84 | 2.1 |
Completeness [%] | 97.2 | 98.2 | 93.5 |
Redundancy | 3.81 | 3.86 | 3.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 310 | 30% PEG 8000, 0.2M MgCl2, 0.1M Tris pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K |