3VPN
Crystal structure of human ribonucleotide reductase subunit M2 (hRRM2) mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-04-04 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 109.082, 109.082, 176.523 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.340 - 2.250 |
R-factor | 0.21335 |
Rwork | 0.212 |
R-free | 0.24671 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3olj |
RMSD bond length | 0.012 |
RMSD bond angle | 1.137 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 4.850 | 2.250 |
Rmerge | 0.074 | 0.033 | 0.692 |
Number of reflections | 51165 | ||
<I/σ(I)> | 10.5 | ||
Completeness [%] | 99.9 | 99.5 | 100 |
Redundancy | 9.6 | 9.2 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 50mM Tris-HCl, 0.1mM KCl, pH 7.5, temperature 293K, VAPOR DIFFUSION, HANGING DROP |