3VPJ
crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa in complex with immune protein Tsi1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-11-27 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 97.000, 97.000, 292.171 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.970 - 2.500 |
| R-factor | 0.2095 |
| Rwork | 0.207 |
| R-free | 0.25740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.059 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.610 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.067 | 0.387 |
| Number of reflections | 56166 | |
| <I/σ(I)> | 21.6 | 5.6 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 9.2 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 20% PEG 4000, 20% isopropanol, 0.1M sodium citrate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
