3VPI

Crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2011-11-14
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	38.450, 63.640, 73.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.610 - 1.500
R-factor	0.1657
Rwork	0.165
R-free	0.18340
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.011
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.610	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.080	0.266
Number of reflections	29544
<I/σ(I)>	14.7	4.8
Completeness [%]	99.4	96
Redundancy	6.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	1.8M sodium formate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	1.8M sodium formate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K