3VPH
L-lactate dehydrogenase from Thermus caldophilus GK24 complexed with oxamate, NADH and FBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.738, 67.843, 147.343 |
| Unit cell angles | 90.00, 94.02, 90.00 |
Refinement procedure
| Resolution | 29.400 - 2.000 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vpg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 96497 | |
| Completeness [%] | 98.7 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | tri-sodium citrate, ammonium phosphate, glycerol, oxamic acid, NADH, fructose 1,6-bisphosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
