3VPG
L-lactate dehydrogenase from Thermus caldophilus GK24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.240, 135.073, 85.877 |
| Unit cell angles | 90.00, 94.44, 90.00 |
Refinement procedure
| Resolution | 29.190 - 1.800 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ldb |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.200 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 111821 | |
| Completeness [%] | 98.2 | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 2-(N-monorpholino)ethanesulfonic acid, polyethyleneglycol 6000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
