3VPA
Staphylococcus aureus FtsZ apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-17 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.485, 81.058, 225.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.489 - 2.487 |
| R-factor | 0.2119 |
| Rwork | 0.210 |
| R-free | 0.25770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vo9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.256 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.487 | 2.500 |
| Rmerge | 0.117 | 0.417 |
| Number of reflections | 47665 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 1.0M Lithium chloride, 0.1M Sodium acetate, 30% PEG 6000, 0.34M Sodium Malonate, pH 7.0, VAPOR DIFFUSION, temperature 293K |
