3VP8
Crystal structure of the N-terminal domain of the yeast general corepressor Tup1p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.170, 43.582, 70.525 |
| Unit cell angles | 90.00, 96.19, 90.00 |
Refinement procedure
| Resolution | 34.585 - 1.910 |
| R-factor | 0.2471 |
| Rwork | 0.244 |
| R-free | 0.29840 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.852 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.600 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 25047 | |
| Completeness [%] | 92.9 | 92.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.2 | 293 | 10%(v/v) 2-propanol, 0.1M phosphate-citrate, 0.2M Li2SO4, pH 4.2, VAPOR DIFFUSION, temperature 293K |
