3VP7
Crystal structure of the beta-alpha repeated, autophagy-specific (BARA) domain of Vps30/Atg6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9670 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.959, 59.959, 113.977 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.000 - 2.300 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.020 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.074 | 0.325 |
| Number of reflections | 9844 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 2.5M sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
