3VOB
Staphylococcus aureus FtsZ with PC190723
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-07-01 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.095, 50.507, 88.288 |
| Unit cell angles | 90.00, 110.95, 90.00 |
Refinement procedure
| Resolution | 32.351 - 2.703 |
| R-factor | 0.1985 |
| Rwork | 0.195 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vo8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.388 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.703 | 2.700 |
| Rmerge | 0.115 | 0.352 |
| Number of reflections | 8237 | |
| <I/σ(I)> | 3.9 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Lithium sulfate, 0.1M Tris-HCl, 25% PEG5000MME, 10% ethylene glycol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
