3VO2
Crystal structure of Zea mays leaf ferredoxin-NADP+ reductase III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-03 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 76.673, 76.673, 108.705 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.330 - 1.390 |
| R-factor | 0.19901 |
| Rwork | 0.198 |
| R-free | 0.21637 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gaw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.211 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.440 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.278 | |
| Number of reflections | 136584 | |
| Completeness [%] | 99.7 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 18% PEG 8000, 0.2M sodium chloride, 0.1M sodium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
