3VNX
Crystal structure of ferritin from multicellular green algae, Ulva pertusa.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 151.875, 151.875, 151.875 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.797 - 2.400 |
| R-factor | 0.1897 |
| Rwork | 0.187 |
| R-free | 0.23920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.998 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.055 | 0.395 |
| Number of reflections | 22816 | |
| <I/σ(I)> | 24.3 | 9.21 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 9.6 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 21% PEG 400, 0.1M Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
