3VNV

Complex structure of viral RNA polymerase II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2011-06-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	139.730, 256.040, 101.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.936 - 2.604
R-factor	0.2227
Rwork	0.220
R-free	0.27940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3agp
RMSD bond length	0.010
RMSD bond angle	1.258
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	55640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	30% PEG400, 0.2M calcium acetate, 0.1M HEPES, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K