3VNM
Crystal structures of D-Psicose 3-epimerase with D-sorbose from Clostridium cellulolyticum H10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-26 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.924, 115.225, 91.614 |
Unit cell angles | 90.00, 105.73, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.120 |
Rwork | 0.190 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vni |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.200 |
High resolution limit [Å] | 2.120 | 2.120 |
Number of reflections | 90274 | |
<I/σ(I)> | 28.4 | 3.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 298 | 0.2M tri-sodium citrate dehydrate, 17-20% Polyethylene Glycol 3350, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |