3VNF

Structure of the ebolavirus protein VP24 from Sudan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97940
Spacegroup name	P 31 2 1
Unit cell lengths	61.110, 61.110, 126.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.030 - 2.100
R-factor	0.2368
Rwork	0.234
R-free	0.27000
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.102
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.030
High resolution limit [Å]	1.800	1.800
Rmerge	0.075
Number of reflections	23986
<I/σ(I)>	8.3
Completeness [%]	95.8	95.8
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	295	0.1M HEPES, pH 7.0, 6% (+/-)-MPD, 15% (w/v) D-(+)-sucrose, VAPOR DIFFUSION, temperature 295K
2	VAPOR DIFFUSION	7	295	0.1M HEPES, pH 7.0, 10% MPD, 1mM DTT, VAPOR DIFFUSION, temperature 295K