3VNC
Crystal Structure of TIP-alpha N25 from Helicobacter Pylori in its natural dimeric form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2009-10-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97898, 0.97917, 0.96395 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.700, 46.936, 99.100 |
| Unit cell angles | 90.00, 127.78, 90.00 |
Refinement procedure
| Resolution | 49.140 - 2.600 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.140 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.102 | 0.374 |
| Number of reflections | 15054 | |
| <I/σ(I)> | 17.7 | 4.7 |
| Completeness [%] | 95.4 | 99.9 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 16% PEG3350, 80% Tacsimate, 2% 1,2-propanediol, 5% MPD, 5% Glycerol, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
