3VMY
Crystal Structure of a parallel coiled-coil dimerization domain from the voltage-gated proton channel (REDUCTION/DTT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2010-05-23 |
Detector | RAYONIX MX225HE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.165, 54.068, 81.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.980 - 1.470 |
R-factor | 0.2 |
Rwork | 0.198 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vmx |
RMSD bond length | 0.023 |
RMSD bond angle | 2.165 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC (5.5.0102) |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.500 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.070 | 0.495 |
Number of reflections | 30985 | |
<I/σ(I)> | 10 | 4.043 |
Completeness [%] | 99.6 | 100 |
Redundancy | 7.2 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 283 | 0.1M KCL, 0.05M TRIS-HCL PH8.0, 0.1M SODIUM MALONATE, 30% PEG 1,000, 5MM DTT, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 283K |