3VMX
Crystal Structure of a parallel coiled-coil dimerization domain from the voltage-gated proton channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-11-22 |
| Detector | RAYONIX MX225HE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.156, 54.010, 81.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.010 - 1.450 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.483 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.056 | 0.440 |
| Number of reflections | 232980 | |
| <I/σ(I)> | 43.972 | 4.741 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 2% V/V TACSIMATE (PH 7.0), 0.1M IMIDAZOLE (PH 7.0), 8% W/V PEG 3350, 5% V/V 2-PROPANOL (PEGRX-2 # 26, HAMPTOM), VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 283K |
