3VMR
Crystal structure of Staphylococcus aureus membrane-bound transglycosylase in complex with moenomycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-21 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 130.423, 52.852, 54.585 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.598 - 3.688 |
R-factor | 0.3036 |
Rwork | 0.303 |
R-free | 0.30920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hzs 3FWM 3fwl |
RMSD bond length | 0.005 |
RMSD bond angle | 1.134 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.598 | 3.830 |
High resolution limit [Å] | 3.680 | 3.688 |
Number of reflections | 4460 | |
Completeness [%] | 99.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 25mM Na malonate, 11.5% PEG3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |