3VMN

Crystal structure of dextranase from Streptococcus mutans

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2007-06-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9700, 0.97888, 0.97931, 0.98400, 0.96400
Spacegroup name	P 1 21 1
Unit cell lengths	53.200, 89.735, 63.258
Unit cell angles	90.00, 102.32, 90.00

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.15292
Rwork	0.151
R-free	0.18179
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.823
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.780	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.063	0.175
Number of reflections	76683
<I/σ(I)>	39.2	11.5
Completeness [%]	100.0	100
Redundancy	7.5	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	30% PEGMME 2000, 0.1M phosphate-citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K