3VM9
Dimeric horse myoglobin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-09 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.800 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.340, 62.523, 83.354 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.050 |
R-factor | 0.128 |
R-free | 0.16800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wla |
RMSD bond length | 0.014 |
RMSD bond angle | 0.030 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.070 |
High resolution limit [Å] | 1.050 | 1.050 |
Rmerge | 0.065 | 0.450 |
Number of reflections | 139129 | |
<I/σ(I)> | 13.2 | 3.1 |
Completeness [%] | 100.0 | 99.5 |
Redundancy | 4.3 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 10% PEG 6000, 200mM CH3COONa, 0.1M Tris-HCl buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |