3VLD

Crystal structure of yeast proteasome interacting protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-16
Detector	Bruker DIP-6040
Wavelength(s)	0.97916, 0.979475, 0.96424
Spacegroup name	P 21 21 21
Unit cell lengths	85.402, 94.676, 129.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.520 - 2.050
R-factor	0.18894
Rwork	0.186
R-free	0.23957
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.081
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.470	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.056	0.345
Number of reflections	66549
<I/σ(I)>	22	4.9
Completeness [%]	99.8	99.5
Redundancy	16.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	8% PEG 3000, 0.2M potassium phosphate, 0.1M Tris-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K