3VKO

Galectin-8 N-terminal domain in complex with sialyllactosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-07-22
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	54.710, 61.500, 84.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.600 - 2.080
Rwork	0.212
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yv8
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.080	2.080
Rmerge	0.061	0.327
Number of reflections	17578
<I/σ(I)>	12.8	4.7
Completeness [%]	99.8	100
Redundancy	5.3	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	30%(v/v) PEG400, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K