3VKI
Monoclinic Crystal Structure of Salmonella FlgA in closed form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.926, 103.317, 85.501 |
| Unit cell angles | 90.00, 107.26, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.2529 |
| Rwork | 0.251 |
| R-free | 0.28120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.969 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (CCP4_3.3.16) |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 39534 | |
| Completeness [%] | 99.3 | 96.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 289 | 12% PEG 6000, 0.05M CITRIC ACID PH 4.2, 1.0M LICl, 14% 2-METHYL-2,4-PENTANEDIOL, vapor diffusion, hanging drop, temperature 289K |
