3VKF
Crystal Structure of Neurexin 1beta/Neuroligin 1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.261, 95.186, 120.396 |
| Unit cell angles | 90.00, 108.62, 90.00 |
Refinement procedure
| Resolution | 48.910 - 3.300 |
| R-factor | 0.21365 |
| Rwork | 0.205 |
| R-free | 0.28690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.046 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.910 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.311 | |
| Number of reflections | 25519 | |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 97.3 | 93.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M sodium chloride, 0.005M calcium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
