3VK1

Green-fluorescent variant of the non-fluorescent chromoprotein Rtms5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-05
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.956600
Spacegroup name	P 42 2 2
Unit cell lengths	93.120, 93.120, 76.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.620 - 2.200
R-factor	0.2071
Rwork	0.205
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mou
RMSD bond length	0.023
RMSD bond angle	1.981
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.2.1)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.620		2.250
High resolution limit [Å]	2.200	9.820	2.200
Rmerge	0.068	0.046	0.967
Rmeas		0.053	1.049
Number of reflections	17621	203	1250
<I/σ(I)>	15.88	32.72	1.91
Completeness [%]	98.9	80.6	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.36M KI, 0.2M TRIS, PH 8.5, 21% PEG 3350, 25% GLYCEROL, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K