3VK0
Crystal Structure of hypothetical transcription factor NHTF from Neisseria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.013, 108.899, 44.083 |
| Unit cell angles | 90.00, 115.61, 90.00 |
Refinement procedure
| Resolution | 27.860 - 1.880 |
| R-factor | 0.1844 |
| Rwork | 0.182 |
| R-free | 0.22991 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b5a |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.574 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.060 | 0.682 |
| Number of reflections | 25883 | |
| <I/σ(I)> | 39.8 | 3.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.72M Sodium formate, 9% PEG8000, 9% PEG1000, 100mM Sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
