3VJP

Orthorhombic Crystal Structure of Salmonella FlgA in closed form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-18
Detector	RAYONIX MX225HE
Wavelength(s)	0.97895
Spacegroup name	C 2 2 21
Unit cell lengths	53.168, 162.502, 103.485
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.545 - 2.700
R-factor	0.2453
Rwork	0.244
R-free	0.27650
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.044
Data reduction software	MOSFLM
Data scaling software	SCALA (CCP4_3.3.9)
Phasing software	PHENIX
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.630	2.850
High resolution limit [Å]	2.700	2.700
Total number of observations		12094
Number of reflections	12420
Completeness [%]	98.7	99.74

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	289	12% PEG 6000, 0.1M CITRIC ACID, 1.0M LiCl, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K