3VIR
Crystal strcture of Swi5 from fission yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.240, 57.899, 64.432 |
| Unit cell angles | 90.00, 96.73, 90.00 |
Refinement procedure
| Resolution | 19.650 - 2.700 |
| R-factor | 0.25378 |
| Rwork | 0.251 |
| R-free | 0.31060 |
| Structure solution method | MIR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.754 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0113) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.058 | 0.263 |
| Number of reflections | 15572 | |
| <I/σ(I)> | 10.4 | 4.5 |
| Completeness [%] | 86.4 | 62.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% MPD, 0.1M Tris-HCl, 24.5mM octyl-b-glucoside, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
