3VIR
Crystal strcture of Swi5 from fission yeast
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-20 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.240, 57.899, 64.432 |
Unit cell angles | 90.00, 96.73, 90.00 |
Refinement procedure
Resolution | 19.650 - 2.700 |
R-factor | 0.25378 |
Rwork | 0.251 |
R-free | 0.31060 |
Structure solution method | MIR |
RMSD bond length | 0.018 |
RMSD bond angle | 1.754 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.6.0113) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.058 | 0.263 |
Number of reflections | 15572 | |
<I/σ(I)> | 10.4 | 4.5 |
Completeness [%] | 86.4 | 62.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% MPD, 0.1M Tris-HCl, 24.5mM octyl-b-glucoside, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |