3VIA
Crystal structure of the PH domain of Evectin-2 from human
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.283, 54.267, 111.638 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.770 - 1.750 |
R-factor | 0.22177 |
Rwork | 0.220 |
R-free | 0.25905 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.073 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.062 | 0.346 |
Number of reflections | 21746 | |
<I/σ(I)> | 48 | 8.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 20% PEG MME 5000, 0.1M bis-tris-HCl, 13% ethylene glycol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |