3VI6
Crystal Structure of human ribosomal protein L30e
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 56.282, 56.282, 203.628 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.590 |
| R-factor | 0.19114 |
| Rwork | 0.190 |
| R-free | 0.21625 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1NUM |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.157 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.620 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.052 | 0.310 |
| Number of reflections | 16978 | |
| <I/σ(I)> | 39 | 8.2 |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 10.7 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.3M potassium chrolide, 1mM EDTA, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
