3VI0
Crystal structure of the O intermediate of the L93A mutant of bacteriorhodopsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 102.300, 102.300, 112.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.300 |
R-factor | 0.288 |
Rwork | 0.288 |
R-free | 0.34810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vhz |
RMSD bond length | 0.008 |
RMSD bond angle | 1.214 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.400 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.113 |
Number of reflections | 18113 |
<I/σ(I)> | 15.1 |
Completeness [%] | 99.8 |
Redundancy | 14.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 283 | membrane fusion method, 2.3 M ammonium sulfate, 0.05M Na-citrate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 283K |