3VHH
Crystal structure of DiMe-biotin-avidin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-18 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.967, 73.510, 81.473 |
| Unit cell angles | 90.00, 119.64, 90.00 |
Refinement procedure
| Resolution | 36.760 - 2.260 |
| R-factor | 0.20297 |
| Rwork | 0.200 |
| R-free | 0.26042 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1avd |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.206 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.096 | |
| Number of reflections | 31246 | |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 2.5-2.7M ammonium sulfate, 0.1M sodium citrate(pH 4.0-5.0), vapor diffusion, sitting drop, temperature 293K |
