3VHF
plant thaumatin I at pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.688, 63.653, 71.743 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.720 - 1.390 |
| R-factor | 0.14898 |
| Rwork | 0.148 |
| R-free | 0.16771 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1thv |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.349 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.390 | 1.400 |
| Rmerge | 0.081 | 0.394 |
| Number of reflections | 40161 | |
| <I/σ(I)> | 57.45 | 5.11 |
| Completeness [%] | 98.7 | 89.4 |
| Redundancy | 9.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 50mM Tris-HCl buffer, 15% PEG3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
