3VH5
Crystal structure of the chicken CENP-T histone fold/CENP-W/CENP-S/CENP-X heterotetrameric complex, crystal form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 137.100, 137.100, 46.548 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.025 - 2.402 |
R-factor | 0.1942 |
Rwork | 0.188 |
R-free | 0.24380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b0b 3b0c |
RMSD bond length | 0.008 |
RMSD bond angle | 1.006 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.025 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.070 | 0.540 |
Number of reflections | 17899 | |
<I/σ(I)> | 30.17 | 4.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.9 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl, 0.16M MgCl2, 0.25M NaCl, 24% PEG4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |