3VH5

Crystal structure of the chicken CENP-T histone fold/CENP-W/CENP-S/CENP-X heterotetrameric complex, crystal form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	137.100, 137.100, 46.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.025 - 2.402
R-factor	0.1942
Rwork	0.188
R-free	0.24380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b0b 3b0c
RMSD bond length	0.008
RMSD bond angle	1.006
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.025	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.070	0.540
Number of reflections	17899
<I/σ(I)>	30.17	4.8
Completeness [%]	99.9	100
Redundancy	9.9	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1M Tris-HCl, 0.16M MgCl2, 0.25M NaCl, 24% PEG4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K