3VGV
E134A mutant nucleoside diphosphate kinase derived from Halomonas sp. 593
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-12 |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 112.165, 92.017, 113.613 |
| Unit cell angles | 90.00, 94.72, 90.00 |
Refinement procedure
| Resolution | 42.950 - 2.500 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nhk |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.474 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.950 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.334 |
| Number of reflections | 73584 | |
| <I/σ(I)> | 9.07 | 1.9 |
| Completeness [%] | 91.9 | 82.2 |
| Redundancy | 2.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2M CALCIUM ACETATE HYDRATE, 0.01M DITHIOTHREITOL (DTT), 0.1M SODIUM CACODYLATE TRIHYDRATE, 18% PEG 8000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
