3VGT
Wild-type nucleoside diphosphate kinase derived from Halomonas sp. 593
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-05 |
| Detector | RIGAKU JUPITER 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.119, 170.640, 77.636 |
| Unit cell angles | 90.00, 93.45, 90.00 |
Refinement procedure
| Resolution | 23.268 - 2.700 |
| R-factor | 0.287 |
| Rwork | 0.287 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nhk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.340 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.268 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.047 | 0.277 |
| Number of reflections | 49180 | |
| <I/σ(I)> | 17.1 | 1.7 |
| Completeness [%] | 91.3 | 82.3 |
| Redundancy | 1.6 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.19M SODIUM ACETATE, 0.09M TRIS HYDROCHLORIDE, 28% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
