3VG7
Structure of human LFABP at high resolution from S-SAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.259, 57.003, 74.664 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.500 - 1.440 |
| R-factor | 0.1645 |
| Rwork | 0.165 |
| R-free | 0.22200 |
| Structure solution method | AB INITIO |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.051 | 0.417 |
| Number of reflections | 24038 | |
| <I/σ(I)> | 103.7 | 9.8 |
| Completeness [%] | 97.3 | 84.6 |
| Redundancy | 2.4 | 23.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 8 | 293 | 30% PEG MME 2000, 0.15M KBr, pH 8.0, hanging drop, temperature 293K |
