3VG6
Barium derivative of human LFABP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-09-10 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.216, 57.240, 74.491 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.220 |
Rwork | 0.209 |
R-free | 0.29530 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.262 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.300 |
High resolution limit [Å] | 2.220 | 4.780 | 2.220 |
Rmerge | 0.068 | 0.054 | 0.257 |
Number of reflections | 6813 | ||
<I/σ(I)> | 16.6 | ||
Completeness [%] | 99.7 | 99.6 | 97.2 |
Redundancy | 13.2 | 11.8 | 12.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 8 | 293 | 30% PEG MME 2000, 0.15M KBr, pH 8.0, hanging drop, temperature 293K |