3VFZ
Crystal structure of -35 promoter binding domain of SigD of Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-01-07 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.430, 35.700, 50.920 |
| Unit cell angles | 90.00, 122.59, 90.00 |
Refinement procedure
| Resolution | 33.883 - 1.901 |
| R-factor | 0.1999 |
| Rwork | 0.198 |
| R-free | 0.23840 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.883 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.062 | 0.588 |
| Number of reflections | 9711 | |
| <I/σ(I)> | 17.9 | 3.4 |
| Completeness [%] | 96.8 | |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 300 | 1.0-1.3M ammonium sulfate, 0.1M Tris-Cl, 5% 2-Methyl-2,4-pentanediol(MPD), 3% Glycerol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
