3VFM

crystal structure of HLA B*3508 LPEP155A, HLA mutant Ala155

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-08
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9536
Spacegroup name	P 21 21 21
Unit cell lengths	50.384, 81.909, 112.256
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.237 - 1.900
R-factor	0.1931
Rwork	0.191
R-free	0.24060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zhk
RMSD bond length	0.006
RMSD bond angle	1.019
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.900
Number of reflections	37187
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.2M ammonium acetate, 16% PEG 4K, 0.1M sodium citrate pH5.6, vapor diffusion, hanging drop, temperature 293K