3VFG
Crystal structure of monoclonal antibody 3F8 Fab fragment that binds to GD2 ganglioside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 116.028, 64.310, 57.241 |
Unit cell angles | 90.00, 93.65, 90.00 |
Refinement procedure
Resolution | 28.580 - 1.650 |
R-factor | 0.17842 |
Rwork | 0.176 |
R-free | 0.22470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aju |
RMSD bond length | 0.020 |
RMSD bond angle | 2.094 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.324 | |
Number of reflections | 49781 | |
<I/σ(I)> | 3 | |
Completeness [%] | 98.4 | 98.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | Hamptom Index D7, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |