3VFC
Crystal structure of enolase MSMEG_6132 (TARGET EFI-502282) from Mycobacterium smegmatis str. MC2 155 complexed with tartrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-09 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 95.264, 95.264, 260.649 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.16081 |
| Rwork | 0.160 |
| R-free | 0.19199 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dvg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.286 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.119 | |
| Number of reflections | 48263 | |
| <I/σ(I)> | 6.1 | 1.7 |
| Completeness [%] | 94.3 | 96.7 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 0.1M TRIS-HCL, pH 8.5, 1.4M AMMONIUM TARTRATE DIBASIC, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
