3VF6
Glucokinase in complex with glucose and activator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-23 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.850, 82.230, 86.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.810 - 1.860 |
R-factor | 0.1773 |
Rwork | 0.176 |
R-free | 0.20470 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Refinement software | BUSTER (2.9.3) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 86.000 |
High resolution limit [Å] | 1.680 |
Number of reflections | 41536 |
Completeness [%] | 96.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20mg/ml protein in 25 mM HEPES pH 7.0, 0.5 mM TCEP, 0.05 M NaCl, 40 mM glucose, 1 mM of activator, Crystallization drops in a 1:1 ratio were set up over wells containing 0.1 M Tris HCl, pH 7.0, 80-200 mM glucose, and 19-26% PEG-4000, VAPOR DIFFUSION, HANGING DROP |