3VEQ
A binary complex betwwen bovine pancreatic trypsin and a engineered mutant trypsin inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-09-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.997, 39.826, 76.783 |
| Unit cell angles | 90.00, 119.65, 90.00 |
Refinement procedure
| Resolution | 19.910 - 2.250 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qyi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | AUTOMAR |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 14218 |
| Completeness [%] | 90.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | VAPOR DIFFUSION, HANGING DROP, temperature 293K |
