3VEO
Crystal structure of the O-carbamoyltransferase TobZ in complex with carbamoyl phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-10-19 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 98.200, 98.200, 279.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.196 - 2.187 |
| R-factor | 0.1665 |
| Rwork | 0.164 |
| R-free | 0.21110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ven |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.072 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.200 | 2.290 | |
| High resolution limit [Å] | 2.187 | 30.000 | 2.187 |
| Rmerge | 0.039 | 0.151 | |
| Number of reflections | 41968 | 4997 | |
| <I/σ(I)> | 46.97 | 15.49 | |
| Completeness [%] | 99.5 | 96.7 | |
| Redundancy | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.6 M sodium potassium tartrate, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
