3VE9

Crystal structure of orotidine 5'-monophosphate decarboxylase from Metallosphaera sedula

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 1 21 1
Unit cell lengths	46.097, 74.870, 55.097
Unit cell angles	90.00, 95.54, 90.00

Refinement procedure

Resolution	45.882 - 1.450
R-factor	0.1943
Rwork	0.193
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ve7
RMSD bond length	0.006
RMSD bond angle	1.014
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.882
High resolution limit [Å]	1.450
Number of reflections	61286
Completeness [%]	93.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	30% PEG 4000, 0.1M sodium acetate, 0.2M ammonium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K