3VE5

Structure of recombination mediator protein RecR16-196 deletion mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	0.9875
Spacegroup name	P 21 21 2
Unit cell lengths	84.384, 84.753, 72.613
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.933 - 2.800
R-factor	0.1958
Rwork	0.194
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vdp
RMSD bond length	0.012
RMSD bond angle	1.458
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Number of reflections	13020

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.15	293	14%(w/v) PEG33500, 100mM Mes, 150mM Ammonium Sulfate, pH 5.15, VAPOR DIFFUSION, HANGING DROP, temperature 293K