3VDX
Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-25 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 122.590, 127.710, 204.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.728 - 3.002 |
| R-factor | 0.2312 |
| Rwork | 0.229 |
| R-free | 0.28130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1BRO 1aa7 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.555 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.730 | 19.730 | 3.080 |
| High resolution limit [Å] | 3.000 | 13.430 | 3.000 |
| Rmerge | 0.206 | 0.044 | 0.669 |
| Number of reflections | 22135 | 273 | 478 |
| <I/σ(I)> | 7.9 | 28.33 | 2.91 |
| Completeness [%] | 68.3 | 65.2 | 20.4 |
| Redundancy | 4.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.3 | 298 | 1.2M NaH2PO4, 0.8M K2HPO4, 0.1M CAPS, pH 10.3, vapor diffusion, hanging drop, temperature 298K |
