3VDU
Structure of recombination mediator protein RecRK21G mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Detector technology | CCD |
Wavelength(s) | 0.9875 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 66.848, 123.729, 135.203 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.724 - 2.800 |
R-factor | 0.2248 |
Rwork | 0.223 |
R-free | 0.25980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vdp |
RMSD bond length | 0.012 |
RMSD bond angle | 1.543 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.700 |
Number of reflections | 6949 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 8%(w/v) PEG4000, 200mM Ammonium Sulfate, 10%(v/v) 2-Propanol, 100mM HEPES Sodium Salt, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |